anti abca1 pe Search Results


abca1 antibody - bsa free  (Bio-Techne corporation)
95
Bio-Techne corporation abca1 antibody - bsa free
Abca1 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
abca1 antibody - bsa free - by Bioz Stars, 2026-08
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anti abca1 antibody pe cy7  (Novus Biologicals)
94
Novus Biologicals anti abca1 antibody pe cy7
Anti Abca1 Antibody Pe Cy7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pm36736225-63-4-7?v=Novus+Biologicals
Average 94 stars, based on 1 article reviews
anti abca1 antibody pe cy7 - by Bioz Stars, 2026-08
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pe-conjugated mouse anti- hla-abc  (Becton Dickinson)
90
Becton Dickinson pe-conjugated mouse anti- hla-abc
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Pe Conjugated Mouse Anti Hla Abc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pmc09880527-78-8-14?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
pe-conjugated mouse anti- hla-abc - by Bioz Stars, 2026-08
90/100 stars
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anti cd68  (Proteintech)
95
Proteintech anti cd68
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Anti Cd68, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pm41747596-98-25-27?v=Proteintech
Average 95 stars, based on 1 article reviews
anti cd68 - by Bioz Stars, 2026-08
95/100 stars
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staining with fitc fluorescein isothiocyanate labeled anti abca1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology staining with fitc fluorescein isothiocyanate labeled anti abca1
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Staining With Fitc Fluorescein Isothiocyanate Labeled Anti Abca1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pmc12775519-72-7-22?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
staining with fitc fluorescein isothiocyanate labeled anti abca1 - by Bioz Stars, 2026-08
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cd163-pe  (Becton Dickinson)
90
Becton Dickinson cd163-pe
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Cd163 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pm35274685-67-45-46?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
cd163-pe - by Bioz Stars, 2026-08
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apc cd11b  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology apc cd11b
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Apc Cd11b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pmc07797913__mmc1-89-6-9?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
apc cd11b - by Bioz Stars, 2026-08
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fitc-conjugated anti-mouse cd14 antibody  (Becton Dickinson)
90
Becton Dickinson fitc-conjugated anti-mouse cd14 antibody
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Fitc Conjugated Anti Mouse Cd14 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pmc04874254-57-35-39?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fitc-conjugated anti-mouse cd14 antibody - by Bioz Stars, 2026-08
90/100 stars
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bs-0024r-pe-cy3  (Bioss)
90
Bioss bs-0024r-pe-cy3
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Bs 0024r Pe Cy3, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/custom%40bs-0024r-pe-cy3%4030389579?v=Bioss
Average 90 stars, based on 1 article reviews
bs-0024r-pe-cy3 - by Bioz Stars, 2026-08
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gapdh  (Abcam)
99
Abcam gapdh
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Gapdh, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/pmc05850208-42-4-43?v=Abcam
Average 99 stars, based on 1 article reviews
gapdh - by Bioz Stars, 2026-08
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bs-0024r-pe-cy7  (Bioss)
90
Bioss bs-0024r-pe-cy7
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Bs 0024r Pe Cy7, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/custom%40bs-0024r-pe-cy7%4030389579?v=Bioss
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bs-0024r-pe  (Bioss)
90
Bioss bs-0024r-pe
iPSCs are vulnerable to complement activation. (A) <t>HLA-ABC</t> surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.
Bs 0024r Pe, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+abca1+pe/custom%40bs-0024r-pe%4030389579?v=Bioss
Average 90 stars, based on 1 article reviews
bs-0024r-pe - by Bioz Stars, 2026-08
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Image Search Results


iPSCs are vulnerable to complement activation. (A) HLA-ABC surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.

Journal: Frontiers in Immunology

Article Title: Inhibition of complement activation by CD55 overexpression in human induced pluripotent stem cell derived kidney organoids

doi: 10.3389/fimmu.2022.1058763

Figure Lengend Snippet: iPSCs are vulnerable to complement activation. (A) HLA-ABC surface protein expression measured by flow cytometry in unstained (US), unstimulated (–) and IFN-γ stimulated (+) iPSCs. A representative histogram is shown of three independent experiments (n=3). (B) mRNA expression of CD46, CD55 and CD59 in iPSCs measured by RT-qPCR (n=3). (C) Complement inhibitor CD46, CD55 and CD59 protein surface expression in iPSCs measured by flow cytometry. Representative histograms are shown of three independent experiments (n=3). (D) Schematic of the experimental procedure to measure complement deposition on iPSCs in vitro . (E) C3 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C3 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C3 + proportion. (F) C5b-9 deposition on iPSCs incubated with heat-inactivated (dNHS) or active normal human serum (NHS) measured by flow cytometry. Results are presented as relative mean fluorescence intensity (MFI) and C5b-9 + proportion. A representative histogram of three independent experiments (n=3) is shown and indicates the gate for the C5b-9 + proportion. Error bars show standard deviation and significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) was evaluated using T-test.

Article Snippet: For flow cytometry the following antibodies were used: PE-conjugated mouse anti- HLA-ABC (1:100, 555553, BD Biosciences), human anti- HLA-A2 antibody (3 µg mL -1 , SN607D8), mouse anti-human CD55 (1:500, ab1422, Abcam), mouse anti-C3 (1:1000, RFK22, in-house generated), mouse anti-C5b-9 (1:100, AE11, Hycult Biotech), PE-conjugated mouse anti-human CD46 (1:100, 12-0469-42, Invitrogen), and APC-conjugated mouse anti-human CD59 (1:100, 17-0596-42, Invitrogen) were used as primary antibodies.

Techniques: Activation Assay, Expressing, Flow Cytometry, Quantitative RT-PCR, In Vitro, Incubation, Fluorescence, Standard Deviation